Coding
Part:BBa_K1916006
Designed by: Jonathan Chen Group: iGEM16_UC_Davis (2016-10-11)
Ga0039498_104087g2 + intein-CBD tag
A GAF domain of the cyanobacteriochrome (CBCR) Ga0039498_104087 with an intein/chitin-binding domain attached to the C-terminus for purification. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 600
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 600
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 576
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 600
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 600
Illegal NgoMIV site found at 1096
Illegal AgeI site found at 1186 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |